Other Tests

xray-insectSeed Moisture Content: This test determines the percentage by weight of moisture content in seeds. Seed moisture content affects the capacity of seeds to stay alive and maintain germinability and vigor over time. *ISTA Accredited Test

Oil and Protein: Using Nuclear Magnetic Resonance Spectrometry (NMR), we can determine oil, protein and moisture content in seeds of various oil crops. The test is non-destructive and the machine can be calibrated for a wide range of crops. Read more.

X-ray: A test to reveal the internal tissues of seeds including any abnormalities, empty seeds, absence of embryos, mechanical and internal damages, and insect infestation. It is particularly useful for native species, trees, shrubs and nuts which can be observed internally without breaking the shell.

fast-green-testChemical tests to detect mechanical damage and seed coat injuries: Fast green, Ferric chloride and Indoxyl acetate tests are used to reveal mechanical injuries and physical fractures in seed coats of corn, soybeans and other seeds.

Test Weight

Seed weight can indicate seed size, density, and the presence of shriveled, small, less developed seeds in a lot. Depending on the variety, production site, and rainfall during the growing season, there can be significant differences in the number of seeds per pound. It helps in determining the seeding rate.

Thermogradient Germination Test 

 

Oil, Protein and Moisture Determination in Seeds Using NMR

The Oregon State University Seed Laboratory proudly announces a new testing service! We can now test seeds for oil, protein and moisture content using NMR spectrometry, a non-destructive technology.

What is NMR is used for?

Nuclear Magnetic Resonance Spectrometry (NMR), is used for determining oil, protein and moisture content in seeds of various oil crops. The instrument is calibrated for several oil crops including soybean, camelina, canola, meadowfoam, and euphorbia, and can be calibrated for other species as needed. Tests can be performed in whole seeds or meals.

The need for NMR

meadowfoam-fieldWith the diversification of crops in Oregon and the search for crops to be used for biodiesel production, the need to determine the oil content of such crops has emerged. In addition, the meal in some oil seeds such as soybean is used for feed after extracting the oil and it is useful to determine the protein content in the meal as well as in the seeds. Both the USDA and Canadian Grain Commission use NMR in determining oil seed content in oil crops.

In the past, researchers have had to send their oil seeds out of the state for oil and protein analysis. The OSU Seed Laboratory’s recent acquisition of a Nuclear Magnetic Resonance Spectrometer means this service is now available in-state. This provides an advantage to Northwest researchers and commercial producers both in testing turnaround and in the availability of the equipment and service for conducting customized research.

The principle of the test

The oil content test estimates the amount of oil that can be extracted from seeds using an industrial extraction method. Direct pressing extracts 90-92%, whereas solvent extraction remove 97-99% of oils contained in seeds.

specialtestingThe NMR technique measures the resonance energy absorbed by hydrogen atoms in the sample. Usually oil contents are expressed based on a specific moisture basis (e.g., 8.5%, 10%, etc.). Using the NMR in measuring oil content is referenced by ISO 10565:1998 Oilseeds.

Sample size and test period

Sample size is 5-10 grams for all analyses (not for each test), i.e., for oil, protein and moisture.  We can calibrate the NMR for a smaller amount of seeds (i.e., 1-2 grams), if needed.   It takes 24 hours to complete the test from the time of receiving the sample. The NMR is a non-destructive method, so seeds can be returned if needed. Please enclose the sample in a moisture-proof (i.e. Ziplock) bag.

Research potential

We welcome inquiries from researchers worldwide. Please feel free to contact us to discuss your research needs.