Having operated continuously since its foundation in 1909 as an official laboratory in a state that produces a broad range of species, the OSU Seed Lab has gradually become a one-stop seed testing place for a rich diversity of species and tests. We use cutting edge equipment and methods for every test and use the most advanced electronic information system to serve our customers. Most of our samples are from within Oregon, but we receive samples from other states and overseas.
Please securely package samples to be tested, include a testing request form, and mail to the lab. Local customers are welcome to drop off samples at the lab; we have an after-hours sample drop box next to the front door. Clients sending live material call for instructions.
We offer a full complement of purity testing services based on both AOSA and ISTA seed testing rules. We can also classify purity results based on schemes used for OECD, HVS, Australia or Canada. Digital imaging is available for any purity component.

AOSA Purity Test This test determines the percentage by weight of pure seed, other crop seeds, inert matter and weed seeds in a test sample. This test can be conducted on commercial, certified, official and other samples. When a customer orders an AOSA purity test, the lab also conducts and reports an all-states noxious weed exam.
AOSA Noxious Weed Exam This test reports the name and number of any noxious weed seeds found in a test sample (minimum 25,000 seeds) based on the Federal all-states noxious-weed seed requirements. A noxious exam for a particular state such as Hawaii or Wisconsin can be performed as well. The test does not report other seeds that are not in the noxious list. The noxious exam is required by the Oregon Certification Program and is automatically conducted when an AOSA purity is ordered.
Undesirable Grass Seeds (UGS) The UGS test is an examination of a test sample (minimum 25,000 seeds) for ten species deemed undesirable in seven eastern states (MD, VA, WV, PA, NJ, NH and DE). These species are bentgrass, bermudagrass, annual bluegrass, rough bluegrass, meadow fescue, tall fescue, orchardgrass, redtop, timothy, and velvetgrass. When marketing turf varieties into the listed states, the seed label must list the name and number per pound or ounce of UGS species found in a test sample under the heading "Noxious Weed Seed" or "Undesirable Grass Seed." This test can be conducted on commercial, certified or official samples.
Oregon Sod Quality Exam (SQ) (Certification eligible samples only.) Search for specific crops and weeds as defined by the program. Sod tests are stopped if disqualifying contaminants or amount of contaminant are found. Please see the Oregon Sod Quality page for more information.
AOSA Crop & Weed Exam (CW) This exam provides a complete listing of all species (both crops and weeds) found in a test sample (minimum 25,000 seeds) and categorizes the seeds as crop seeds or weed seeds. Both the actual number of seeds found in a test sample (e.g., 1 seed in 50g) and the rate of occurrence (number of seeds per pound) are reported. Because the CW test provides complete listing of all the seeds found in a test sample, Oregon Sod Quality and UGS reports can be generated in most cases without testing the sample again, saving time and money for customers. This test can be conducted on commercial, certified, official or other samples. It is required for Certified AOSA/EC basic and pre-basic tagging, Canada testing and WA Sod Quality.
ISTA Purity Test This test determines the percentage by weight of pure seed, other seeds and inert matter. The minimum sample size is 2500 seeds (See the ISTA International Rules for Seed Testing for more details). ISTA purity tests can be performed only on species listed in the ISTA Rules. *ISTA Accredited Test
ISTA Other Seed Determination (OSD) (also called Determination of Other Seeds by Number) This test determines the number and identity of other seeds in a test sample. The extent of this determination is referred to as the scope of the test. *ISTA Accredited Test The scope depends on customer needs:
Soil Exam Determines the percentage of soil particles in the working sample. Can be performed as a sequential test, i.e. other tests will be done only if soil exam results are within a certain specification.
Pest and Disease Exam The working sample is examined for a list of pests and diseases. Affected seeds are removed, identified, weighed and reported by percentage.
Orobanche Test Determines the presence of Orobanche seeds in a submitted sample of red clover using a dry method. This test requires its own sample.
Ergot A test to detect Ergot (Claviceps purpurea) in a sample. The ergoted seeds are removed, weighed and reported as percentage.
Friction Separation Exam Certified alfalfa and red clover samples must be tested using a dodder mill. The material removed is examined for dodder or any other Oregon prohibited weeds. A 500g bulk examination is required for certification purposes.
Noxious/Prohibited Tests for Other Countries A test performed at customer request. The noxious weeds list varies with the country. The customer must provide a current list of noxious/prohibited weeds of the particular country.
Germination A physiological viability test which determines the percentage of live seeds that produce normal seedlings under favorable germination conditions. Both AOSA and ISTA require 400 seeds for germination in most cases. *ISTA Accredited Test
Tetrazolium (TZ) A quick biochemical viability test which determines the number of live seeds lot based on dehydrogenase activity in seeds. It indicates the percentage of live and dead seeds in any sample regardless of its dormancy level. The test can be performed in 24-48 hours. *ISTA Accredited Test
Fluorescence Test for Ryegrass This test requires germinated seedlings. Determines the fluorescence level of any particular ryegrass sample. It is used to estimate a potential contamination of perennial ryegrass with annual ryegrass. This test is performed on all AOSA ryegrass germinations and is used to differentiate Annual from Perennial ryegrass under AOSA rules. It is performed on ISTA samples by request and is not used in ISTA purity calculations. *ISTA Accredited Test
Cold Test (CT): A vigor test that indicates the potential emergence of seeds under early spring field conditions where cold and wet soils are prevalent. Used for corn, beans, peas, and vegetables that are planted in the early spring in cold-wet soil.
Accelerated Aging (AAT): A vigor test, where seeds are stressed with high temperature and relative humidity for a period of time prior to the germination test. High quality seeds tolerate such stress conditions and retain their capabilities to produce normal seedlings when germinated.
Conductivity Test (CT): A test to measure the integrity of the cell membranes. Low vigor seeds have leaky membrane structure and release electrolytes into the soak-water. The level of electrolytes is reflected in high electric conductivity of the soak-water.
Seedling Growth Rate (SGR): At the end of the standard germination test, the dry matter of the seedlings is determined using the oven method. Seedling dry matter was found to correlate closely with vigor (AOSA Seed Vigor Testing Handbook).
Speed of Germination (SG): high quality seeds germinate faster than poor quality seeds. The number of normal seedlings recorded in the first count represents the population of fast germinating seeds and thus functions as a vigor measurement (AOSA Seed Vigor Testing Handbook). The following formula is used to calculate the speed of germination (SG):
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Ploidy by Cytometry: A test separating diploid and tetraploid ryegrass genotypes, or any genotypes with different chromosome numbers based on the amount of DNA in the nucleus of plant cells. This test can be performed on germinated seedlings or leaf tissue from the greenhouse or field. Currently it is being used for research projects in a broad range of crops.
Fluorescence Test for Ryegrass: Determines the fluorescence level of any particular ryegrass sample. It is used to estimate a potential contamination of perennial ryegrass with annual ryegrass. This test is performed on all AOSA ryegrass germinations and is used to calculate AOSA purity results. It is performed on ISTA samples by request and is not used in ISTA purity calculations. *ISTA Accredited Test
Ammonia Fluorescence Test for Fine Fescue: A quick test to distinguish between Festuca ovina and Festuca rubra. The roots of F. rubra fluoresce yellow and the roots of F. ovina fluoresce green when sprayed with at 0.5% solution of ammonium hydroxide. This test is performed on all AOSA fine fescue germination tests. It is performed on ISTA samples by request. *ISTA Accredited Test
Fluorescence Grow-out for Ryegrass: A test involves growing fluorescent ryegrass seedlings using a specific protocol. At the end of the prescribed test period, the plants are evaluated according to their growth habits. The data is used to make AOSA purity calculations as to the percentage of perennial and annual ryegrass in a sample. Growout results may be reported on an ISTA certificate but the results are not used in ISTA purity calculations.
Varietal Fluorescence Level (VFL): This test is required by the National Grass Variety Review Board before releasing a new ryegrass variety describing its inherited fluorescence level or to re-describe an existing variety. It involves fluorescence tests of at least 3000 seeds and a grow-out of the fluorescing seedlings following a specific protocol.
Grow-out of Other Species: A test in which seedlings are grown in greenhouse conditions until they develop characteristics that allow them to be differentiated. This is applicable to a wide range of species and is useful in conditions where normal purity determinations cannot be made with certainty.

Clearfield Wheat Bioassay: Determine the percentage of the “Clearfield®” trait (a herbicide tolerant trait) in a population of 400 seed sample that represents a seed lot. The test is completed in approximately 10 days.

Sodium Hydroxide Test for Wheat (NaOH): A chemical test used to distinguish between red and white wheat in situations where it is difficult to distinguish between the two types (i.e. when seeds have been weather-damaged, treated, or are simply hard to distinguish by the naked eye).
Phenol Test: A quick test for cultivar identification. It is based on color reaction between phenol solution and seed coat (pericarp). It can be used for wheat, barley, oat, ryegrass and bluegrass. When a test sample is compared with a pure control check, it can be determined if the sample is pure or mixed with other cultivars.
Endophyte: A test to detect the presence or absence of endophyte fungi (Neotyphodium spp.) in seeds of various grass species such as tall fescue and ryegrass. It helps growers to make decisions regarding the marketing of grass seeds for feed or turf purposes.
Pest and Disease (P&D): The working sample is examined for a list of pests and diseases. Affected seeds are removed, identified, weighed and reported by percentage.
Orobanche Test (OT): Determines the presence of Orobanche seeds in a submitted sample of red clover using a dry method.
Ergot: A test to detect Ergot (Claviceps purpurea) in a sample. The ergoted seeds are removed, weighed and reported as percentage.
The Oregon Sod Quality Program is managed by the Oregon Department of Agriculture Commodity Inspection Division and requires that an Oregon Certification eligible sample should pass through three parts: 1) Purity and noxious exam; 2) Oregon Sod Quality exam, and 3) viability test (germination or TZ). Experimental varieties are not eligible for Sod Quality tagging. Sod quality tests are not standardized throughout the USA, so other states may have different sod test requirements. The text of the Oregon Administrative Rule that authorizes the sod program and governs standards is included below.
OAR 603-056-0145
Standards for Sod Quality Certification
(1) The seed lots submitted shall meet all standards for certification by Oregon State University.
(2) The seed standards for "Oregon Sod Quality Seed" of hard fescue, sheep fescue, blue fescue, Kentucky bluegrass, red fescue, chewings fescue, perennial ryegrass, bentgrass and tall fescue shall be as set out in Table 1.
(3) "Noxious Weed" and "Crop and Weed" analyses shall be based on a 20 gram sample for hard fescue, sheep fescue and blue fescue, 25 gram sample for Kentucky bluegrass (except a 10 gram Poa annua search), 30 gram sample for red fescue and chewings fescue, 50 gram sample for ryegrass and tall fescue, and a 2-1/2 gram sample for bentgrass. Testing shall be discontinued when results of the tests exceed the maximum limits set forth in Table 1.
| Kind | Minimum purity |
Minimum germination | Maximum other crop *1 | Maximum weed *5 |
| perennial ryegrass | 98 % | 90 % | 0.10 % *2 | 0.02 % |
| marion Kentucky bluegrass | 95 % | 80 % | 0.10 % *3 | 0.02 % |
| other varieties of bluegrass | 97 % | 80 % | 0.10 % *3 | 0.02 % |
| red fescue | 98 % | 90 % | 0.10 % | 0.02 % |
| chewings fescue | 98 % | 90 % | 0.10 % | 0.02 % |
| hard fescue | 98 % | 85 % | 0.10 % | 0.02 % |
| sheep fescue | 98 % | 85 % | 0.10 % | 0.02% |
| blue fescue | 98 % | 85 % | 0.10 % | 0.02 % |
| bentgrass | 98 % | 85 % | 0.10 % *4 | 0.10 % |
| tall fescue | 98.5 % | 85 % | 0.10 % | 0.02 % |
Australia is an important market for seeds produced in Oregon and the USA. Recent weather conditions in Australia may result in more seed being needed there. If your seed is going to be headed for Australia, it is important to know their requirements and how the OSU Seed Laboratory can help you.
The OSU Seed Laboratory is ISTA accredited and AQIS approved and can issue ISTA certificates and analysis reports that are accepted by AQIS. (See AQIS Import Condition C7180 for more information.)
If Glyceria spp. seeds are found, knowing that your seed is headed for Australia, the OSU Seed Laboratory can quickly proceed to test the Glyceria by flow cytometry.
Australia requires a special classification scheme for reporting purity results. Whether you need an ISTA Orange Certificate, ISTA Blue Certificate, or OSU analysis report, the test results will be classified according to Australia's requirements.
The OSU Seed Laboratory can issue ISTA Orange and Blue certificates or standard OSU analysis reports on the samples, depending on customer requirements and the species being tested. Please contact us if you have questions.
ISTA Certificates are mailed to the customer. Standard OSU analysis reports are provided via our website, fax or remote printing for those that already have this service.
Our hope is that these seed testing and reporting services help you meet the requirements of your customers and Australian authorities.
Seed Moisture Content: This test determines the percentage by weight of moisture content in seeds. Seed moisture content affects the capacity of seeds to stay alive and maintain germinability and vigor over time. *ISTA Accredited Test
Oil and Protein: Using Nuclear Magnetic Resonance Spectrometry (NMR), we can determine oil, protein and moisture content in seeds of various oil crops. The test is non-destructive and the machine can be calibrated for a wide range of crops. Read more.
X-ray: A test to reveal the internal tissues of seeds including any abnormalities, empty seeds, absence of embryos, mechanical and internal damages, and insect infestation. It is particularly useful for native species, trees, shrubs and nuts which can be observed internally without breaking the shell.
Chemical tests to detect mechanical damage and seed coat injuries: Fast green, Ferric chloride and Indoxyl acetate tests are used to reveal mechanical injuries and physical fractures in seed coats of corn, soybeans and other seeds.
Test Weight
Seed weight can indicate seed size, density, and the presence of shriveled, small, less developed seeds in a lot. Depending on the variety, production site, and rainfall during the growing season, there can be significant differences in the number of seeds per pound. It helps in determining the seeding rate.
The Oregon State University Seed Laboratory proudly announces a new testing service! We can now test seeds for oil, protein and moisture content using NMR spectrometry, a non-destructive technology.
Nuclear Magnetic Resonance Spectrometry (NMR), is used for determining oil, protein and moisture content in seeds of various oil crops. The instrument is calibrated for several oil crops including soybean, camelina, canola, meadowfoam, and euphorbia, and can be calibrated for other species as needed. Tests can be performed in whole seeds or meals.
With the diversification of crops in Oregon and the search for crops to be used for biodiesel production, the need to determine the oil content of such crops has emerged. In addition, the meal in some oil seeds such as soybean is used for feed after extracting the oil and it is useful to determine the protein content in the meal as well as in the seeds. Both the USDA and Canadian Grain Commission use NMR in determining oil seed content in oil crops.
In the past, researchers have had to send their oil seeds out of the state for oil and protein analysis. The OSU Seed Laboratory’s recent acquisition of a Nuclear Magnetic Resonance Spectrometer means this service is now available in-state. This provides an advantage to Northwest researchers and commercial producers both in testing turnaround and in the availability of the equipment and service for conducting customized research.
The oil content test estimates the amount of oil that can be extracted from seeds using an industrial extraction method. Direct pressing extracts 90-92%, whereas solvent extraction remove 97-99% of oils contained in seeds.
The NMR technique measures the resonance energy absorbed by hydrogen atoms in the sample. Usually oil contents are expressed based on a specific moisture basis (e.g., 8.5%, 10%, etc.). Using the NMR in measuring oil content is referenced by ISO 10565:1998 Oilseeds.
Sample size is 5-10 grams for all analyses (not for each test), i.e., for oil, protein and moisture. We can calibrate the NMR for a smaller amount of seeds (i.e., 1-2 grams), if needed. It takes 24 hours to complete the test from the time of receiving the sample. The NMR is a non-destructive method, so seeds can be returned if needed. Please enclose the sample in a moisture-proof (i.e. Ziplock) bag.
We welcome inquiries from researchers worldwide. Please feel free to contact us to discuss your research needs.
The goal of the OSU Seed Lab is to provide accurate, timely and cost effective seed testing services. We value our customers and strive to keep costs as low as possible while maintaining our cutting edge high quality services. Fees reflect the amount of work needed for each test. All prices are in US dollars.
Purity, Germination & TZ | Bulk Exams | Other Tests | Other Services
| AOSA purity includes noxious examination. ISTA purity includes ISTA certificate (Blue or Orange). Please consult our ISTA information before ordering ISTA tests. Samples requiring extra steps or those with high levels of impurities (other crops, weeds, multiple florets, inert materials) requiring more than 1 hour analyst time will be charged a prorated hourly charge based on extra time required. 10% discount on germination retests ordered Jan-June. ISTA TZ test requires double the amount of seeds. |
|||
| Species | Purity | Germination | TZ |
| Grasses | |||
|
Bentgrass (Agrostis sp) (purity incl. species sep.) |
$92.00 |
$43.00 |
$62.00 AOSA |
|
Bluegrass (Poa sp) |
$80.00 |
$45.00 |
|
|
Tall fescue (Festuca arundinacea) |
$62.00 |
$41.00 |
|
|
Fine fescue (other Festuca sp) (germ incl. ammonia test) |
$66.00 |
$42.00 |
|
|
Orchardgrass (Dactylis glomerata) |
$63.00 |
$40.00 |
|
|
Annual ryegrass (Lolium multiflorum) (germ incl. fluorescence) |
$47.00 |
$41.00 |
|
|
Perennial ryegrass (Lolium perenne) (germ incl. fluorescence) |
$69.00 |
$46.00 |
|
|
Mixture (per each additional kind) |
$56.00 |
$39.00 |
|
|
Native species and other grasses |
hourly |
$51.00 |
|
| Cereals, Legumes, Vegetables, Flowers | |||
|
Cereals (wheat, barley, oat, etc) |
$49.00 |
$38.00 |
$62.00 AOSA |
|
Alfalfa, Clover |
$47.00 |
$35.00 |
|
|
Bean, pea, corn, melon, pumpkin, squash, cucumber |
$38.00 |
$35.00 |
|
|
Beet, sugar and table (add $44 if sprout count requested) |
$49.00 |
$65.00 |
|
|
Brassicas |
$77.00 |
$35.00 |
|
|
Carrot, celeriac, celery, dill, parsley, parsnip |
$45.00 |
$35.00 |
|
|
Chive, leek, onion, spinach, okra |
$37.00 |
$35.00 |
|
|
Eggplant, lettuce, pepper, tomato |
$45.00 |
$35.00 |
|
|
Flowers |
hourly |
varies |
|
| Trees and Shrubs | |||
|
Trees and Shrubs - (**20% germination discount January-May) |
$38.00 |
$50.00** |
$62.00 AOSA |
|
ISTA additional strat (add'l 400 seeds req. for certain species) |
|
$44.00 |
|
|
AOSA multiple stratification (add'l 200 seeds) |
|
$22.00 |
|
|
100 seed germination test (any tree/shrub species) |
|
$36.00 |
|
|
Test or Service (please consult ISTA information to determine which tests can be reported on an ISTA Certificate) |
Price |
|
Seed Moisture |
$38.00 |
|
Seed Weight (incl. 1000 seed weight, number of seeds per pound) |
$28.00 |
|
Ploidy by cytometry for Ryegrass |
$115.00 |
|
Ploidy by cytometry for Glyceria sp. (mannagrass) |
$40.00 - $115.00 |
| Protein, oil and water analysis - example: one sample tested only for protein is $10, for protein and oil is $20, for protein, oil and water is $30. | $10.00/test/sample |
|
X-ray (AOSA only) |
$21.00 |
|
Endophye test (immunoblot assay - 100 seeds) |
$110.00 |
|
Varietal identification tests (Phenol, NaOH, etc for wheat, barley, oat, ryegrass, bluegrass) |
$51.00 |
|
Clearfield Wheat bioassay |
$59.00 |
|
Vigor tests (cold test, accelerated aging, conductivity, etc) |
$53.00 |
| Seedling vigor classification test (beans) | $53.00 |
| Sprout count (beets) | $60.00 |
|
Broomrape (Orobanche minor) in red clover and other species (dry method) |
$95.00 |
| Fluorescence test on ryegrass contaminants found in purity tests. Fee varies by number of seeds grown. $20 minimum up to 10 seeds, $30 for up to 15 seeds, more than 15 seeds: $46 maximum. | $20.00 - $46.00 |
|
Grow-out of fluorescent ryegrass seedlings, up to 40 fluorescent seedlings (about 10% test fluorescence) |
$199.00 |
|
Grow-out of ryegrass, additional fluorescent seedlings |
hourly |
| Grow-out, non-ryegrass (based on number of seedlings to be grown out) | $50.00 - $199.00 |
| Grow-out, crops/weed seed contaminants found in tests (varies by number/type of seeds, $20 minimum/test) | $20.00 - $300.00 |
|
Varietal Fluorescence Level (VFL) in perennial ryegrass, three lots |
$940.00 |
| VFL, annual ryegrass, three lots | $1090.00 |
| VFL, additional charge for varieties fluorescing over 10% | $150.00 |
|
VFL, each additional lot |
$126.00 |
|
Cereal grow-out (to distinguish winter from spring varieties) |
$50.00 - $150.00 |
| Ammonia test for Red and Hard fescue | $42.00 |
| Mill check | $56.00 |
|
Soil seed-bank (search for seeds in soil, compost, etc) |
$150.00 |
| Toxicity test for soil and compost | $63.00 |
|
Noxious weed search in animal feed pellets |
hourly |
|
Noxious weed search in bird seeds |
hourly |
| Search for foreign materials | $71.00 |
| Special handling charge for unusual samples | $20.00 |
|
Digital image by email of requested contaminants (subject to hourly charge; varies according to number and size of contaminants and complexity of image) |
$30.00 - $150.00 |
|
Research projects, specialized and custom testing |
call |
| Hourly charge (applies to especially contaminated or extraordinary samples; also used for custom work such as sample preparation or special bulk searches) | $56.00/hr |
|
Service |
Price |
|
Rush (purity results in 24-48 hours) |
$100.00 |
|
Priority Rush (call first) |
$150.00 |
|
Produce UGS or Sod report from existing CW |
$22.00 |
|
Revision of report (example - correction of customer error on sample certificate) |
$10.00 |
|
Conversion of report (paperwork only) requiring no additional testing more info |
$36.00 |
|
Conversion of report requiring additional testing more info |
$52.00 |
| EC supplemental report for certification | $10.00 |
|
Additional copy of report |
$10.00 |
|
ISTA Blue or Orange Certificate - duplicate or provisional |
$10.00 |
|
ISTA Blue or Orange Certificate - original if no purity ordered |
$10.00 |
|
Cancellation of tests - fee is proportional to work completed prior to cancellation |
$16.00 min. |
| Surcharge on out of country samples (e.g. to cover wire transfer fees) |
$22.00 |
Numbers represent those samples (new + retests) that are in the purity testing process.
| Date Sample or Test Request Received |
Remaining Samples |
| Rush & Super Rush Samples | 0 |
| May 22 | 3 |
| May 23 | 7 |
| Total | 10 |
Number of samples completed by the purity unit yesterday (May 23) including rushes: 10
Crop and weed exams can be converted to SQ, UGS or Canada certificates in most cases. A crop and weed exam on Tall fescue can be converted to SQ, UGS or Canada certificate with no additional testing required.
SQ can be converted to UGS certificates in most cases. A completed sod quality report on Tall fescue can be converted to an UGS certificate with no additional testing required.
AOSA/EC to AOSA: Paperwork only
AOSA to AOSA/EC: Paperwork only
CONVERSION WITH ADDITIONAL TESTING REQUIRED: